The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FGF10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FGF10. Next,...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of, biological activities toward cells of mesenchymal, neuronal, and epithelial origin. FGF10 belongs to the subgroup of FGFs that also includes FGF3, 7, and 22 (1). Mature mouse FGF10 is an approximately 20kD protein that...

Fibroblast Growth Factor 10 (FGF10) BioAssay(TM) ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of FGF10 in mouse serum, plasma and other biological fluids. Detection Range: 15.6-1,000pg/ml, Sensitivity: 5.3pg/ml, Storage and Stability: Desiccation recommended. The Standard,...